解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The currently used multistep chemical synthesis for making surfaces antimicrobial by attaching to them hydrophobic polycations is replaced herein by an aerosol-assisted plasma deposition procedure. To this end, N,N-hexyl,methyl-PEI (HMPEI) is directly plasma-coated onto a glass surface. The resultant immobilized HMPEI...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0593-4
更新日期:2014-02-01 00:00:00
abstract::This study shows that electroanalysis may be used in vanillin biotechnological production. As a matter of fact, vanillin and some molecules implicated in the process like eugenol, ferulic acid, and vanillic acid may be oxidized on electrodes made of different materials (gold, platinum, glassy carbon). By a judicious c...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0631-2
更新日期:2014-02-01 00:00:00
abstract::Stachybotrys microspora is a filamentous fungus characterized by the secretion of multiple hydrolytic activities (cellulolytic and non-cellulolytic enzymes). The production of these biocatalysts was studied under submerged culture using glucose, cellulose, and wheat bran as carbon sources. Endoglucanases, pectinases, ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0608-1
更新日期:2014-02-01 00:00:00
abstract::The capability to grow microalgae in nonsterilized wastewater is essential for an application of this technology in an actual industrial process. Batch experiments were carried out with the species in nonsterilized urban wastewater from local treatment plants to measure both the algal growth and the nutrient consumpti...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0629-9
更新日期:2014-02-01 00:00:00
abstract::Proteases are some of the most important industrial enzymes, and one of their main applications is for the production of cheese in the dairy industry. Due to a shortage of animal rennet, microbial coagulant proteases are being sought. In this work, the production of microbial rennet from Thermomucor indicae-seudaticae...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0655-7
更新日期:2014-02-01 00:00:00
abstract::The present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of Azadirachta indica. Improvement in the azadirachtin production via triggering its biosynthetic pathway in plant cells was carried out by the exogenous addition of prec...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0664-6
更新日期:2014-02-01 00:00:00
abstract::Internal air circulation affects the temperature field distribution in a gas double-dynamic solid-state fermentation bioreactor (GDSFB). To enhance heat transfer through strengthening internal air circulation in a GDSFB, we put an air distribution plate (ADP) into the bioreactor and studied the effects of forced inter...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0670-8
更新日期:2014-02-01 00:00:00
abstract::Here, we demonstrate the micropropagation protocol of Argyrolobium roseum (Camb.), an endangered herb exhibiting anti-diabetic and immune-suppressant properties, and antioxidant enzymes pattern is evaluated. Maximum callogenic response (60 %) was observed from leaf explant at 1.0 mg L(-1) 1-nephthalene acetic acid (NA...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0591-6
更新日期:2014-01-01 00:00:00
abstract::Using microalgae to capture CO2 from flue gas is an ideal way to reduce CO2 emission, but this is challenged by the high cost of carbon capture and transportation. To address this problem, a bicarbonate-based integrated carbon capture and algae production system (BICCAPS) has been proposed, in which bicarbonate is use...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0515-5
更新日期:2014-01-01 00:00:00
abstract::Xylans are major hemicellulose components of plant cell wall which can be hydrolyzed by xylanolytic enzymes. Three forms of endo-β-1,4-xylanases (XynSW1, XynSW2A, and XynSW2B) produced by thermotolerant Streptomyces sp. SWU10 have been reported. In the present study, we described the expression and characterization of...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0508-4
更新日期:2014-01-01 00:00:00
abstract::Fungi had become the main resource of polyunsaturated fatty acids, especially linoleic acid. The research studied the effects and mechanism of selenium on polyunsaturated fatty acids of Diasporangium jonesianum. The results showed that selenium could significantly increase the yields of linoleic acid. In contrast, the...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0477-7
更新日期:2014-01-01 00:00:00
abstract::The bacterium Enterobacter A47 has demonstrated the ability to synthesise distinct exopolysaccharides (EPS) as a function of the substrate used. The culture's performance was evaluated in experiments using either glucose or xylose, as single carbon sources, and compared with the substrate (glycerol) used in previous s...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0560-0
更新日期:2014-01-01 00:00:00
abstract::The present study deals with the characterization of halotolerant protease produced by Bacillus aquimaris VITP4 strain isolated from Kumta coast, Karnataka, India. The studies were performed at 40 °C and pH 8 in Tris buffer. Metal ions such as Mn(2+) and Ca(2+) increased the proteolytic activity of the enzyme by 34 an...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0524-4
更新日期:2014-01-01 00:00:00
abstract::L-Ribose is an important precursor for antiviral agents, and thus its high-level production is urgently demanded. For this aim, immobilized recombinant Escherichia coli cells expressing the L-arabinose isomerase and variant mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans were developed. The im...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0547-x
更新日期:2014-01-01 00:00:00
abstract::The importance of using tissue-specific promoters in the genetic transformation of plants has been emphasized increasingly. Here, we report the isolation of a novel seed-specific promoter region from peanut and its validation in Arabidopsis and tobacco seeds. The reported promoter region referred to as groundnut seed ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0482-x
更新日期:2014-01-01 00:00:00
abstract::The decrease in toxicity of carbon capture reclaimer monoethanolamine (MEA) waste (MEAw) during anaerobic degradation of such waste together with easily degradable organics was investigated. Samples were collected from a bioreactor at steady state with 86 % organic chemical oxygen demand removal at room temperature, w...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0587-2
更新日期:2014-01-01 00:00:00
abstract::Ornithine carbamoyltransferase folding/unfolding is a complex and not completely understood process. Our experimental results suggest that ornithine carbamoyltransferase interacts in a completely different way with urea and guanidine hydrochloride. In fact, we noticed that, increasing concentration from 0.0 to 8.0 M o...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0580-9
更新日期:2014-01-01 00:00:00
abstract::The ability of non-living biomass of an arsenic-hypertolerant Bacillus cereus strain SZ2 isolated from a gold mining environment to adsorb As (III) from aqueous solution in batch experiments was investigated as a function of contact time, initial As (III) concentration, pH, temperature and biomass dosage. Langmuir mod...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0490-x
更新日期:2013-12-01 00:00:00
abstract::Enzymatic polymerization of phenolic compounds (catechol, resorcinol, and hydroquinone) was carried out using laccase. The mechanism of polymerization and the structures of the polymers were evaluated in terms of UV-Vis and Fourier transform infrared spectroscopy. The molecular weights of the produced polyphenols were...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0463-0
更新日期:2013-12-01 00:00:00
abstract::Although the use of lipases as biocatalysts has frequently been proposed, it is yet scarcely being implemented in industrial processes. This is mainly due to the difficulties associated with the discovery and engineering of new enzymes and the lack of versatile screening methods. In this study, we screened the availab...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0464-z
更新日期:2013-12-01 00:00:00
abstract::Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase (SOD) and catalase (CAT) were successfully synthesized using double emulsion (w/o/w) solvent evaporation technique. Characterization of the nanosphere using dynamic light scattering, field emission scanning electron microscope, and Four...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0427-4
更新日期:2013-12-01 00:00:00
abstract::An anaerobic sequencing batch biofilm reactor (AnSBBR-total volume 7.5 L; liquid volume 3.6 L; treated volume per cycle 1.5 L) treated sucrose-based wastewater to produce biohydrogen (at 30 °C). Different applied volumetric organic loads (AVOL of 9.0, 12.0, 13.5, 18.0, and 27.0 kg COD m(-3) day(-1)), which were varied...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0457-y
更新日期:2013-12-01 00:00:00
abstract::Agricultural by-products are becoming an attractive substrate for bioethanol production. The aim of this study was to evaluate the effects of regional differences in the rice hulls using Escherichia coli KO11 for bioethanol production. The rice hulls coded Edirne were obtained from Thrace Region, and the rice hulls co...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0504-8
更新日期:2013-12-01 00:00:00
abstract::Bacillus amyloliquefaciens K103 isolated from a lemon sample was used as a biocontrol agent to suppress Rhizoctonia solani Kühn and other fungal plant pathogens. Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance li...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0424-7
更新日期:2013-12-01 00:00:00
abstract::Microalgae farming has been identified as the most eco-sustainable solution for producing biodiesel. However, the operation of full-scale plants is still limited by costs and the utilization of industrial and/or domestic wastes can significantly improve economic profits. Several waste effluents are valuable sources of...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0481-y
更新日期:2013-12-01 00:00:00
abstract::Waste frying oil, which not only harms people's health but also causes environmental pollution, can be a good alternative to partially substitute petroleum diesel through transesterification reaction. This oil contained 8.8 % of free fatty acids, which cause a problem in a base-catalyzed process. In this study, synthe...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0449-y
更新日期:2013-12-01 00:00:00
abstract::A new bioprocess for production of sorbitol and gluconic acid from two low-cost feedstocks, inulin and cassava starch, using a commercially available enzyme was proposed in this study. The commercial glucoamylase GA-L NEW from Genencor was found to demonstrate a high inulinase activity for hydrolysis of inulin into fr...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0278-z
更新日期:2013-12-01 00:00:00
abstract::Chemical composition of Larrea tridentata leaves was determined and elevated content of lignin (35.96 % w/w) was found. The present study was proposed in order to evaluate the extraction of bioactive compounds, particularly phenolic compounds, by solid-state fermentation (SSF) of L. tridentata leaves. The basidiomycet...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0222-2
更新日期:2013-11-01 00:00:00
abstract::A semi-pilot continuous process (SPCP) for enzymatic biodiesel synthesis utilizing near-critical carbon dioxide (NcCO₂) as the reaction medium was developed with the aim of reducing the reaction time and alleviating the catalyst inhibition by methanol. Biodiesel synthesis was evaluated in both lab-scale and semi-pilot...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0189-z
更新日期:2013-11-01 00:00:00
abstract::The effect of biosurfactant rhamnolipid (RL) on hydrolysis and acidification of waste activated sludge (WAS) was investigated. The results indicated that RL could greatly reduce the surface tension of sludge, resulting in stimulating the hydrolysis rate of WAS and enhancing the production of short-chain fatty acids (S...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0439-0
更新日期:2013-11-01 00:00:00
abstract::The terminal monosaccharide of glycoconjugates on a eukaryotic cell surface is typically a sialic acid (Neu5Ac). Increased sialylation usually indicates progression and poor prognosis of most carcinomas. Here, we utilize two human mammary epithelial cell lines, HB4A (breast normal cells) and T47D (breast cancer cells)...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0409-6
更新日期:2013-10-01 00:00:00
abstract::Qualitative and quantitative DNA-based methods were applied to detect genetically modified foods in samples from markets in the Kingdom of Saudi Arabia. Two hundred samples were collected from Al-Qassim, Riyadh, and Mahdina in 2009 and 2010. GMOScreen 35S and NOS test kits for the detection of genetically modified org...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0405-x
更新日期:2013-10-01 00:00:00
abstract::Aspergillus niger phytase (PhyA) has been used as a feed supplement to improve the bioavailability of phytate phosphorus to swine and poultry. However, it is unable to maintain its stability due to high temperature during the feed pelleting process. In this study, we performed site-directed mutagenesis in the Aspergil...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0380-2
更新日期:2013-10-01 00:00:00
abstract::Regulation of the cysteine protease activity is imperative for proper functioning of the various organ systems. Elevated activities of cysteine proteinases due to impaired regulation by the endogenous cysteine proteinase inhibitors (cystatins) have been linked to liver malignancies. To gain an insight into these regul...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0383-z
更新日期:2013-10-01 00:00:00
abstract::Bacillus amyloliquefaciens LL3 is a glutamate-independent poly-γ-glutamic acid (γ-PGA) producing strain which consists of a circular chromosome (3,995,227 bp) and an endogenous plasmid pMC1 (6,758 bp). The study of the function of native plasmid and the genome-size reduction of the B. amyloliquefaciens LL3 strain requ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0382-0
更新日期:2013-09-01 00:00:00
abstract::NADP(+)-dependent isocitrate dehydrogenase from Yarrowia lipolytica CLIB122 (YlIDP) was overexpressed and purified. The molecular mass of YlIDP was estimated to be about 81.3 kDa, suggesting its homodimeric structure in solution. YlIDP was divalent cation dependent and Mg(2+) was found to be the most favorable cofacto...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0373-1
更新日期:2013-09-01 00:00:00
abstract::Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subti...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0325-9
更新日期:2013-09-01 00:00:00
abstract::In this work, fibronectin purification from human plasma with the gelatin-immobilised poly(hydroxyethyl methacrylate) (PHEMA) cryogel has been evaluated. The PHEMA cryogel was prepared by cryo-polymerisation which proceeds in an aqueous solution of monomer frozen inside a plastic syringe. The PHEMA cryogel contained i...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0352-6
更新日期:2013-09-01 00:00:00
abstract::Hydrogen (H2) production from the organic fraction of solid waste such as fruit and vegetable waste (FVW) is a novel and feasible energy technology. Continuous application of this process would allow for the simultaneous treatment of organic residues and energy production. In this study, batch experiments were conduct...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0341-9
更新日期:2013-09-01 00:00:00
abstract::Sugarcane bagasse samples were pretreated with ozone via atmospheric O2 pressure plasma. A delignification efficiency of approximately 80 % was observed within 6 h of treatment. Some hemicelluloses were removed, and the cellulose was not affected by ozonolysis. The quantity of moisture in the bagasse had a large influ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0362-4
更新日期:2013-09-01 00:00:00